Saturday, June 18, 2011

Try Animal Studies. GSPE (grape seed proanthocyanidin extract) Preventing Doxorubicin Kardiotoksis Effects

Doxorubicin is one of the antitumor chemotherapeutic drug works and has been widely used. However, clinical application is very limited because kardiotoksisitas effects associated with cumulative dose. The mechanism causing such effects are expected kardiotosisitas the process of oxidative stress mediated by reactive oxygen species (ROS). Doxorubicin increases intracellular ROS production that causes significant cell death and decreased redox ratio of reduced glutathione (GSH) / oxidized glutathione (GSSG) and disruption of mitochondrial membrane potential.

Studies in animals try one of them conducted by Yalcin, E. et al have been published in the Journal of Medicinal Food in 2010 showed that grape seed proanthocyanidin extract (GSPE), can reduce the formation of ROS induced by doxorubicin and may protect cardiomyocytes from oxidant injury induced by doxorubicin,
then conducted a study using primary cultured cardiomyocytes treated with doxorubicin (Dox, 10 mikroM) alone or GSPE (50 mikrog / mL) with doxorubicin 10 mikroM for 24 hours.

Agarose gel electrophoresis analysis showed that nuclear DNA damage caused by doxorubicin. GSPE treatment while pressing the change. Electron Spin Resonance spectroscopy data show that GSPE hydroxyl radicals with a powerful and radical DPPH. These findings suggest that GSPE in combination with doxorubicin had a protective effect against toxicity induced by doxorubicin in cardiomyocytes, as part of the antioxidant activity. The combination of doxorubicin with GSPE also does not reduce the effect of doxorubicin in inhibiting cell proliferation of human breast carcinoma MCF-7.

Hence, GSPE may be a promising adjunct therapy to prevent kardiotoksisitas without affecting the antineoplastic activity during chemotherapy with doxorubicin.

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